Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
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|red | |red | ||
− | | | + | | Ethanol |
| adhE | | adhE | ||
| CTTAACGCACTCGTAGAGCGTGTAAA | | CTTAACGCACTCGTAGAGCGTGTAAA | ||
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|red | |red | ||
− | | | + | | Ethanol |
− | | | + | | ack (indirectly, pta) |
− | | | + | | GTTTTTTTAGCCACGTATCAATTAT |
|- | |- | ||
|orange | |orange | ||
− | | | + | |Ethanol |
− | | | + | |ldhA |
− | | | + | |ATTCAACATCACTGGAGAAAGTCTT |
− | + | ||
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|blue | |blue |
Latest revision as of 18:39, 16 October 2018
Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | Ethanol | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
green | E | LdhA | ttgtgctataaacggcgagtttcat |
blue | E | pta | ttcacgacaacgttcaataatcat |
pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
purple | Acetate | adhE | GATTTACTAAAAAAGTTTAACATTATC |
white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT |
orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT |
blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |