Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
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(→T/R) |
(→Fermentation product and gene targeted:) |
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Line 10: | Line 10: | ||
|- | |- | ||
|red | |red | ||
− | | | + | | Ethanol |
− | | | + | | adhE |
− | | | + | | CTTAACGCACTCGTAGAGCGTGTAAA |
|- | |- | ||
|orange | |orange | ||
− | | | + | |Ethanol |
− | | | + | |pta-ack |
− | | | + | |CTATGGCTCCCTGACGTTTT |
|- | |- | ||
|yellow | |yellow | ||
− | | | + | |Ethanol |
− | | | + | |frdA |
− | | | + | |GCTGTGGGATAAAAACAATCTGGAG |
|- | |- | ||
|green | |green | ||
− | | | + | |E |
− | | | + | |LdhA |
− | | | + | |ttgtgctataaacggcgagtttcat |
|- | |- | ||
|blue | |blue | ||
− | | | + | |E |
− | | | + | |pta |
− | | | + | |ttcacgacaacgttcaataatcat |
|- | |- | ||
|pink | |pink | ||
− | | | + | |Acetate |
− | | | + | |adhE |
− | | | + | |TTACTAAAAAAGTTTAACATTATCA |
|- | |- | ||
|purple | |purple | ||
− | | | + | |Acetate |
− | | | + | |adhE |
− | | | + | |GATTTACTAAAAAAGTTTAACATTATC |
|- | |- | ||
|white | |white | ||
|Acetate | |Acetate | ||
− | | | + | | adhE |
− | | | + | |CTGATAATGTTAAACTTTTT |
|} | |} | ||
Line 59: | Line 59: | ||
|- | |- | ||
|red | |red | ||
− | | | + | | Ethanol |
− | | | + | | ack (indirectly, pta) |
− | | | + | | GTTTTTTTAGCCACGTATCAATTAT |
|- | |- | ||
|orange | |orange | ||
− | | | + | |Ethanol |
− | | | + | |ldhA |
− | | | + | |ATTCAACATCACTGGAGAAAGTCTT |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
|- | |- | ||
|blue | |blue | ||
− | | | + | | Ethanol |
− | | | + | | ackA |
− | | | + | | TTTTTAGCCACGTATCAATTAT |
|} | |} |
Latest revision as of 18:39, 16 October 2018
Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | Ethanol | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
green | E | LdhA | ttgtgctataaacggcgagtttcat |
blue | E | pta | ttcacgacaacgttcaataatcat |
pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
purple | Acetate | adhE | GATTTACTAAAAAAGTTTAACATTATC |
white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT |
orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT |
blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |