Difference between revisions of "20.109(F24):Fall 2024 schedule"

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| [[20.109(F24):M1D5 | Treat cells for CometChip assay]]<br>  
 
| [[20.109(F24):M1D5 | Treat cells for CometChip assay]]<br>  
| [[20.109(F24):Homework#Due_M1D5|Homework due]] <br> [[20.109(F24):Research talk| <font color =  #962c17 >'''Research talk due'''</font color>]] XXX at 10 pm
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| [http://be.mit.edu/directory/bevin-p-engelward BPE] <br>  
 
| [[20.109(F24):M1D6 | Image and analyze data for CometChip assay]]<br>  
 
| [[20.109(F24):M1D6 | Image and analyze data for CometChip assay]]<br>  
| [[20.109(F24):Homework#Due_M1D6|Homework due]]  
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| [[20.109(F24):Homework#Due_M1D6|Homework due]] <br> [[20.109(F24):Research talk| <font color =  #962c17 >'''Research talk due'''</font color>]] Mon, Sep 30 at 10 pm
 
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Latest revision as of 01:40, 18 June 2024

20.109(F24): Laboratory Fundamentals of Biological Engineering

Fa24 banner image.png

Fall 2024 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Genomic integrity        M2: Protein engineering        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sep 5/6 NLL
Orientation and laboratory tour
M1D1 T/W Sep 10/11 BPE
Learn best practices for mammalian cell culture
Orientation quiz
Homework due
M1D2 R/F Sep 12/13 BPE
Prepare and treat cells for γH2AX experiment
Homework due
M1D3 T/W Sep 17/18 BPE
Use immunoflourescence staining to assess γH2AX experiment
Homework due
R/F Sep 19/20 BE Comm Lab Career Day holiday
M1D4 T/W Sep 24/25 BPE
Complete data analysis for γH2AX experiment
Laboratory quiz
Homework due
M1D5 R/F Sep 26/27 BPE
Treat cells for CometChip assay
Homework due
M1D6 T/W Oct 1/2 BPE
Image and analyze data for CometChip assay
Homework due
Research talk due Mon, Sep 30 at 10 pm
M1D7 R/F Oct 3/4 BPE
Examine experimental data using statistical methods
Laboratory quiz
Homework due
M2D1 T/W Oct 8/9 BCM
Determine peptide design strategy
Homework due
M2D2 R/F Oct 10/11 BCM
Clone cell surface peptide display plasmid
Homework due
T/W Oct 15/16 Indigenous Peoples' Day holiday Data Summary draft due XXX at 10 pm
M2D3 R/F Oct 17/18 AB
Sequence clones and transform into yeast
Homework due
Data Summary revision due XXX at 10 pm
M2D4 T/W Oct 22/23 AB
Align sequencing and stain yeast for flow cytometry
Laboratory quiz
Homework due
R/F Oct 24/25 Journal article presentations
T/W Oct 29/30 Journal article presentations
M2D5 R/F Oct 31/Nov 1 BCM
Analyze flow cytometry and harvest cells to test cadmium sequestration
Homework due
M2D6 T/W Nov 5/6 AB
Assess quality of cadmium sulfide particles through fluorimetry
Homework due
M2D7 R/F Nov 7/8 AB
Visualize cadmium sulfide sequestration through TEM
Laboratory quiz
Homework due
T/W Nov 12/13 Veterans' Day holiday
M3D1 R/F Nov 14/15 []
Brainstorm ideas for Research proposal presentation
Homework due
M3D2 T/W Nov 19/20 AB
Pitch research proposal presentation ideas
M3D3 R/F Nov 21/22 BE Comm Lab Develop ideas for Research proposal presentation
Homework due
T/W Nov 26/27 Thanksgiving Day holiday Research article due XXX at 10 pm
R/F Nov 28/29 Thanksgiving Day holiday
M3D4 T/W Dec 3/4 []
Participate in Research proposal peer reviews
Homework due
R/F Dec 5/6 Research proposal presentations
T Dec 10 Celebration lunch!