Difference between revisions of "20.109(F17): Notes for M1"
From Course Wiki
(→M1D1) |
(→M1D2) |
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Line 18: | Line 18: | ||
Per group, aliquot | Per group, aliquot | ||
*in main lab | *in main lab | ||
− | ** | + | **1x PBS glass bottle |
− | **2 eppendorf tubes of | + | **2 eppendorf tubes of LMP agarose (in eppendorf water bath) |
− | **large scheme of the plate so they can keep track of how to load chips | + | **print a large scheme of the plate so they can keep track of how to load chips |
+ | |||
*and separately, in the TC hood, | *and separately, in the TC hood, | ||
− | ** | + | **5 mL 1x PBS |
− | ** | + | **10 mL MEF media |
− | + | ||
− | + | ||
− | + | ||
*4 binder clips | *4 binder clips | ||
*1 glass slide | *1 glass slide | ||
Line 34: | Line 33: | ||
*sharp jar | *sharp jar | ||
*pipet aid | *pipet aid | ||
− | + | ||
*team sticker to identify glass slides | *team sticker to identify glass slides | ||
*printout of today's protocol | *printout of today's protocol | ||
*large scheme of the plate to hang in TC so they can keep track of how to load chips | *large scheme of the plate to hang in TC so they can keep track of how to load chips |
Revision as of 17:44, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of DMEM media
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
M1D2
Per group, aliquot
- in main lab
- 1x PBS glass bottle
- 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
- print a large scheme of the plate so they can keep track of how to load chips
- and separately, in the TC hood,
- 5 mL 1x PBS
- 10 mL MEF media
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- team sticker to identify glass slides
- printout of today's protocol
- large scheme of the plate to hang in TC so they can keep track of how to load chips