Difference between revisions of "Spring 11:Directed Evolution/Week of 4-18-2011 Status"
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==Monday Evening== | ==Monday Evening== | ||
− | * | + | * 2 Cultures of I13521 started from freezer stocks tubes 2&3. Aliquoted into 5mL of LB+Amp and left to grow overnight. ~12 hours of growth time should be sufficient for experimentation tomorrow. |
− | + | * Idea: potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the ''fliC273'' gene. | |
=Notes= | =Notes= |
Revision as of 06:06, 19 April 2011
Weekly Progress
Weekend/Monday
- Finalized parts list.
- Continued coding image analysis / segmentation code.
- Fabricated Microfluidics using resources from Kamm lab and Voldemann lab.
- PDMS poured & cured Monday afternoon
- Moved microscope setup to larger breadboard.
Monday Evening
- 2 Cultures of I13521 started from freezer stocks tubes 2&3. Aliquoted into 5mL of LB+Amp and left to grow overnight. ~12 hours of growth time should be sufficient for experimentation tomorrow.
- Idea: potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the fliC273 gene.
Notes
Biology
- David Liu has agreed to give us MP-QUR, pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid.
- The Elowitz Repressilator can apparently be found on Add-Gene, will need to either find or figure out a good testing rig to put it in.
- Cory has I13521 and I13522, although both are in pSB1A3, which might prove problematic if the KAF95 fliC is also on a pBR322 derived plasmid. Current backup is J04450 which can be found in pSB1C3 and would allow for a co-transformant.