Difference between revisions of "Spring 11:Directed Evolution/Week of 4-18-2011 Status"

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Revision as of 20:47, 18 April 2011

Weekend/Monday

Initial chambers for testing cells & fluorescent beads
  • Finalized parts list.
  • Continued coding image analysis / segmentation code.
  • Fabricated Microfluidics using resources from Kamm lab and Voldemann lab.
  • Moved microscope setup to larger breadboard.


Notes

Biology

  • David Lu has agreed to give us MP-QUR, pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid.
  • The Elowitz Repressilator can apparently be found on Add-Gene, will need to either find or figure out a good testing rig to put it in.
  • Cory has I13521 and I13522, although both are in pSB1A3, which might prove problematic if the KAF95 fliC is also on a pBR322 derived plasmid. Current backup is J04450 which can be found in pSB1C3 and would allow for a co-transformant.