Difference between revisions of "20.109(S14): Spring 2014 schedule"

From Course Wiki
Jump to: navigation, search
Line 99: Line 99:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14):Introduction to system components (Day1)| Introduction to system components]]
 
|
 
|
 
|--
 
|--
Line 107: Line 107:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14):Cell plating and plasmid digestion (Day2)| Cell plating and plasmid digestion]]
 
|
 
|
 
|--
 
|--
Line 115: Line 115:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14): Begin Western protein analysis (Day3)| Begin Western protein analysis]]
 
|
 
|
 
|--
 
|--
Line 123: Line 123:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14):Complete Western and plasmid purification (Day4)| Complete Western and plasmid purification]]
 
|
 
|
 
|--
 
|--
Line 136: Line 136:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14):Cell preparation for DNA repair analysis (Day5)| Cell preparation for DNA repair analysis]]
 
|
 
|
 
|--
 
|--
Line 144: Line 144:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14):DNA repair assays (Day6)| DNA repair assays]]
 
|
 
|
 
|--
 
|--
Line 152: Line 152:
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
| [http://web.mit.edu/be/people/samson.shtml LS]
 
|
 
|
|
+
| [[20.109(S14):Data analysis (Day7)| Data analysis]]
 
|
 
|
 
|--
 
|--

Revision as of 14:42, 17 January 2014


20.109(S14): Laboratory Fundamentals of Biological Engineering

Feliks signaling-network-crop.jpg

Home        Schedule Spring 2014        Assignments       
Module 1        Module 2        Module 3              

MODULE DAY DATE LECTURER TA LAB NOTES
Tue/Wed Feb 4/5 ANS Orientation
1 1 Thur/Fri Feb 6/7 JR Microbial DNA extraction lab orientation quiz
1 2 Tue/Wed Feb 11/12 JR Diagnostic primer design
1 3 Thur/Fri Feb 13/14 JR 16S PCR and paper discussion
President's Day Feb 17 No lab Feb 18 or Feb 19
1 4 Thur/Fri Feb 20/21 JR DNA cloning
1 5 Tue/Wed Feb 25/26 JR DNA sequencing
1 6 Thur/Fri Feb 27/28 JR Journal club I
1 7 Tue/Wed Mar 4/5 JR Phylogenetic and primer analyses
1 8 Thur/Fri Mar 6/7 JR Journal club II
2 1 Tue/Wed Mar 11/12 LS Introduction to system components
2 2 Thur/Fri Mar 13/14 LS Cell plating and plasmid digestion
2 3 Tue/Wed Mar 18/19 LS Begin Western protein analysis
2 4 Thur/Fri Mar 20/21 LS Complete Western and plasmid purification
Spring Break Mar 24-28 No lab all week
2 5 Tue/Wed Apr 1/2 LS Cell preparation for DNA repair analysis
2 6 Thur/Fri Apr 3/4 LS DNA repair assays
2 7 Tue/Wed Apr 8/9 LS Data analysis
3 1 Thur/Fri Apr 10/11 ANS Design experiment
3 2 Tue/Wed Apr 15/16 ANS Initiate cell culture
3 3 Thur/Fri Apr 17/18 ANS Testing cell viability
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
3 4 Thu/Fri Apr 24/25 ANS Preparing cells for analysis
Tue/Wed Apr 29/30 No lecture or lab today.
3 5 Thur/Fri May 1/2 ANS Initiating transcript and protein assays
3 6 Tue/Wed May 6/7 ANS Transcript-level analysis
3 7 Thur/Fri May 8/9 ANS Protein-level and wrap-up analysis
Module 3 Summary Due
3 8 Tue/Wed May 13/14 ANS Student presentations
Thur May 15 No lab session. Meet in TBD from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.