Difference between revisions of "20.109(S14):Initiating transcript and protein assays (Day5)"
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There are several ways to assess protein concentration, either quantitatively or as present above some threshold value. In the second module, you used one approach to identifying a specific protein that is only one among many in a complex mixture: Western blot. In this third module, you will use an approach that is somewhat easier to make quantitative: ELISA. | There are several ways to assess protein concentration, either quantitatively or as present above some threshold value. In the second module, you used one approach to identifying a specific protein that is only one among many in a complex mixture: Western blot. In this third module, you will use an approach that is somewhat easier to make quantitative: ELISA. | ||
− | As you may recall, a great way to identify a specific protein from a complex mixture is to exploit antibodies | + | As you may recall, a great way to identify a specific protein from a complex mixture is to exploit antibodies |