Difference between revisions of "20.109(S11):Start-up biomaterials engineering (Day1)"
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#While you are waiting, you can add 1 ml of sterile 0.1% gelatin to the two leftmost wells of a six-well dish (one dish per pair) and also label the dish. | #While you are waiting, you can add 1 ml of sterile 0.1% gelatin to the two leftmost wells of a six-well dish (one dish per pair) and also label the dish. | ||
#*The gelatin will be removed before you seed your MES cells but it is important to pre-treat the dish this way. MES must grow on either a "feeder layer" of fibroblasts, or on a gelatin-coated surface. '''The pre-treatment must be done for at least 10 minutes.''' | #*The gelatin will be removed before you seed your MES cells but it is important to pre-treat the dish this way. MES must grow on either a "feeder layer" of fibroblasts, or on a gelatin-coated surface. '''The pre-treatment must be done for at least 10 minutes.''' | ||
− | #*Label the plate lid with your group colour, | + | #*Label the plate lid with your group colour, today s date, and the cell line (called [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=SCRC-1010&Template=cellBiology |