Difference between revisions of "20.109(F08): Fall 2008 schedule"

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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08):Start-up genome engineering | DNA engineering using PCR]]
+
| [[20.109(F08): Mod 1 Day 1 DNA engineering using PCR | DNA engineering using PCR]]
 
|  
 
|  
 
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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): Agarose gel electrophoresis | Clean and cut DNA]]
+
| [[20.109(F08): Mod 1 Day 2 Clean and cut DNA | Clean and cut DNA]]
 
|  
 
|  
 
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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): DNA ligation and bacterial transformation| Agarose gel electrophoresis]]
+
| [[20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis| Agarose gel electrophoresis]]
 
|  
 
|  
 
|--
 
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| [[http://web.mit.edu/be/people/engelward.htm BE]
 
| [[http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): Examine candidate clones| DNA ligation and bacterial transformation]]
+
| [[20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations| DNA ligation and bacterial transformation]]
 
|  
 
|  
 
|--
 
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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): M13.1| Examine candidate clones]]
+
| [[20.109(F08): Mod 1 Day 5 Examine candidate clones| Examine candidate clones]]
 
|  
 
|  
 
|--
 
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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): Western analysis| Restriction map and tissue culture]]
+
| [[20.109(F08): Mod 1 Day 6 Restriction map and tissue culture | Restriction map and tissue culture]]
 
|  
 
|  
 
|--
 
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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): Probe western| Lipofection]]
+
| [[20.109(F08): Mod 1 Day 7 Lipofection| Lipofection]]
−
|  
+
| your first draft of the Mod 1 lab report is due before lab today
 
|--
 
|--
 
| 1
 
| 1
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| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
 
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F08): Module 1 oral presentations| FACS analysis]]
+
| [[20.109(F08): Mod 1 Day 8 FACS analysis| FACS analysis]]
 
|  
 
|  
 
|--
 
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): siRNA design| Protein engineering with PCR]]
+
| [[20.109(F08): Mod 2 Day 1 Protein engineering with PCR| Protein engineering with PCR]]
−
|  
+
| your Powerpoint pitch is due before lab today
 
|--
 
|--
 
| 2
 
| 2
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): Transfection| Yeast transformation]]
+
| [[20.109(F08): Mod 2 Day 2 Yeast transformation| Yeast transformation]]
 
|  
 
|  
 
|--
 
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): Luciferase assays and RNA prep| Colony PCR, Journal article discussion]]
+
| [[20.109(F08): Mod 2 Day 3 Colony PCR and journal article discussion| Colony PCR, Journal article discussion]]
 
|
 
|
 
|--
 
|--
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): Journal article discussion| SDS-PAGE, screen for phenotypes]]
+
| [[20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes| SDS-PAGE, screen for phenotypes]]
 
| Guest lecture: [[User:Nlerner| NL]] from the writing program.  
 
| Guest lecture: [[User:Nlerner| NL]] from the writing program.  
 
|-
 
|-
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): Probe Western| Probe Western, isolate RNA]]
+
| [[20.109(F08): Mod 2 Day 5 Probe western, isolate RNA| Probe Western, isolate RNA]]
 
|  
 
|  
 
|--
 
|--
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): Journal Club I| Journal Club I]]
+
| [[20.109(F08): Mod 2 Day 6 Journal Club I| Journal Club I]]
 
|  
 
|  
 
|--
 
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): cDNA synthesis and microarray| cDNA synthesis and microarray]]
+
| [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray| cDNA synthesis and microarray]]
 
|  
 
|  
 
|--
 
|--
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| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
 
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F08): Module 2 oral presentations| Microarray data analysis]]
+
| [[20.109(F08): Mod 2 Day 8 Microarray data analysis| Microarray data analysis]]
 
|  
 
|  
 
|--
 
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Growth of phage materials| Phage growth]]
+
| [[20.109(F08): Mod 3 Day 1 Growth of phage materials| Phage growth for materials]]
−
|  
+
| your first draft of your research article is due before lab today
 
|--
 
|--
 
| 3
 
| 3
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Phage nanowires| Phage nanowires]]
+
| [[20.109(F08): Mod 3 Day 2 Phage nanowires| Phage nanowires]]
 
|  
 
|  
 
|--
 
|--
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Transmission electron microscopy| Journal Club II]]
+
| [[20.109(F08): Mod 3 Day 3 Journal Club II| Journal Club II]]
 
|  
 
|  
 
|--
 
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Phage by design| Phage by design]]
+
| [[20.109(F08): Mod 3 Day 4 Phage by design| Phage by design]]
 
|  
 
|  
 
|--
 
|--
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Phage by design, pt2| Phage by design, pt 2]]
+
| [[20.109(F08): Mod 3 Day 5 Phage by design, pt2| Phage by design, pt 2]]
 
|  
 
|  
 
|--
 
|--
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): ECD assembly| ECD assembly]]
+
| [[20.109(F08): Mod 3 Day 6 ECD assembly| ECD assembly]]
 
|  
 
|  
 
|--
 
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| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
 
|[http://openwetware.org/wiki/Luis_Alvarez LA]
 
|[http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Module 3 oral presentations| Oral presentations]]
+
| [[20.109(F08): Mod 3 Day 7 Oral presentations| Oral presentations]]
 
|  
 
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Revision as of 02:30, 24 July 2008


20.109(F08): Laboratory Fundamentals of Biological Engineering

20.109(F08)pic.jpg

Home        People        Schedule Fall 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Thur Sept 4 NK Orientation
1 1 Tue Sept 9 BE MS DNA engineering using PCR
1 2 Thur Sept 11 BE MS Clean and cut DNA
1 3 Tue Sept 16 BE MS Agarose gel electrophoresis
1 4 Thur Sept 18 [BE MS DNA ligation and bacterial transformation
1 5 Tue Sept 23 BE MS Examine candidate clones
1 6 Thur Sept 25 BE MS Restriction map and tissue culture
1 7 Tue Sept 30 BE MS Lipofection your first draft of the Mod 1 lab report is due before lab today
1 8 Thur Oct 2 BE MS FACS analysis
2 1 Tues Oct 7 NK BB Protein engineering with PCR your Powerpoint pitch is due before lab today
2 2 Thur Oct 9 NK BB Yeast transformation
2 3 Tues Oct 14 NK BB Colony PCR, Journal article discussion
2 4 Thur Oct 16 NK BB SDS-PAGE, screen for phenotypes Guest lecture: NL from the writing program.
2 5 Tues Oct 21 NK BB Probe Western, isolate RNA
2 6 Thurs Oct 23 NK BB Journal Club I
2 7 Tues Oct 28 NK BB cDNA synthesis and microarray
2 8 Thurs Oct 30 NK BB Microarray data analysis
3 1 Tues Nov 4 AB LA Phage growth for materials your first draft of your research article is due before lab today
3 2 Tue Nov 6 AB LA Phage nanowires
Veteran's Day Break Nov 10,11 No lab Tuesday and Wednesday
3 3 Thur Nov 13 AB LA Journal Club II
3 4 Tue Nov 18 AB LA Phage by design
3 5 Thur Nov 20 AB LA Phage by design, pt 2
Thanksgiving Break Nov No lab or lecture all week
3 6 Tue Dec 2 AB LA ECD assembly
3 7 Thur Dec 4 AB LA Oral presentations
3 8 Tue Dec 9 end of term celebration... Lecture, no lab, for class evaluations then lunch from noon-1:30 PM,
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