Difference between revisions of "20.109(S08): TA notes for module 1"
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(New page: {{Template:20.109(S08)}} ==General notes== ==Daily Notes== === Day 1===) |
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===[[20.109%28S08%29:DNA_engineering/DNA_engineering_by_PCR | Day 1]]=== | ===[[20.109%28S08%29:DNA_engineering/DNA_engineering_by_PCR | Day 1]]=== | ||
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+ | Lab orientation quiz (not a TA quiz) will be taken today. | ||
+ | |||
+ | Materials required: | ||
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+ | #PCR Master Mix (2.5X), ~ 50 μL per group | ||
+ | #DI water, prep 100 μL aliquot per group | ||
+ | #5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev | ||
+ | # 2 PCR tubes per group | ||
+ | |||
+ | Keep everything cold! |
Revision as of 19:06, 27 December 2007
General notes
Daily Notes
Day 1
Lab orientation quiz (not a TA quiz) will be taken today.
Materials required:
- PCR Master Mix (2.5X), ~ 50 μL per group
- DI water, prep 100 μL aliquot per group
- 5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev
- 2 PCR tubes per group
Keep everything cold!