Difference between revisions of "20.109(F07): M13.1 and MDS titers"
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===Green Team=== | ===Green Team=== | ||
*Team members: Arjun Naskar, Patrick Alayon | *Team members: Arjun Naskar, Patrick Alayon | ||
− | *Description of M13.1 phage: | + | *Description of M13.1 phage: p3F17Ag |
*Description of MDS strain: MDS60 | *Description of MDS strain: MDS60 | ||
*Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen. | *Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen. | ||
Line 98: | Line 98: | ||
===Purple Team=== | ===Purple Team=== | ||
− | *Team members: | + | *Team members:Phillip Samayoa, Justin Tan |
− | *Description of M13.1 phage: | + | *Description of M13.1 phage: p7p9 unstuff |
− | *Description of MDS strain: | + | *Description of MDS strain: MDS41 |
*Any notes about titers presented here: no phage control has plaques | *Any notes about titers presented here: no phage control has plaques | ||
Line 113: | Line 113: | ||
|- | |- | ||
| 10-6 | | 10-6 | ||
− | | | + | |~500 |
− | | | + | | ND |
− | | | + | |20 |
− | | | + | |5 |
|- | |- | ||
| 10-8 | | 10-8 | ||
− | | | + | |20 |
− | | | + | |ND |
− | | | + | |3 |
− | | | + | |5 |
|- | |- | ||
| 10-10 | | 10-10 | ||
− | | | + | |~100 |
− | | | + | |ND |
− | | | + | |0 |
− | | | + | |5 |
|} | |} | ||
</center> | </center> | ||
Line 199: | Line 199: | ||
===Pink Team=== | ===Pink Team=== | ||
− | *Team members: | + | *Team members:Roshini Zachariah and Juliana Rotter |
− | *Description of M13.1 phage: | + | *Description of M13.1 phage: p3F17Ag |
− | *Description of MDS strain: | + | *Description of MDS strain: MDS60 |
− | *Any notes about titers presented here: | + | *Any notes about titers presented here:negative control had no plaques as expected |
+ | ====Plaque numbers==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS | ||
+ | |- | ||
+ | | 10-6 | ||
+ | |~100 | ||
+ | |ND | ||
+ | |0 | ||
+ | |0 | ||
+ | |- | ||
+ | | 10-8 | ||
+ | |0 | ||
+ | |ND | ||
+ | |0 | ||
+ | |0 | ||
+ | |- | ||
+ | | 10-10 | ||
+ | |0 | ||
+ | |ND | ||
+ | |0 | ||
+ | |ND | ||
+ | |} | ||
+ | </center> | ||
+ | ====Titer==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS | ||
+ | |- | ||
+ | | titer (PFU/ml) | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |} | ||
+ | </center> | ||
+ | |||
===Blue Team=== | ===Blue Team=== | ||
− | *Team members: | + | *Team members: Augusto Tentori and Jessica Keenan |
*Description of M13.1 phage: | *Description of M13.1 phage: | ||
*Description of MDS strain: | *Description of MDS strain: | ||
Line 254: | Line 299: | ||
===Yellow Team=== | ===Yellow Team=== | ||
− | *Team members: | + | *Team members:Rebecca Kusko and Jessie Wang |
− | *Description of M13.1 phage: | + | *Description of M13.1 phage:p8BAP |
− | *Description of MDS strain: | + | *Description of MDS strain: MDS60 |
*Any notes about titers presented here: | *Any notes about titers presented here: | ||
+ | ====Plaque numbers==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS | ||
+ | |- | ||
+ | | 10-6 | ||
+ | |~100 | ||
+ | | ND | ||
+ | |0 | ||
+ | |0 | ||
+ | |- | ||
+ | | 10-8 | ||
+ | |9 | ||
+ | |ND | ||
+ | |0 | ||
+ | |0 | ||
+ | |- | ||
+ | | 10-10 | ||
+ | |0 | ||
+ | |ND | ||
+ | |0 | ||
+ | | 1 | ||
+ | |} | ||
+ | </center> | ||
+ | ====Titer==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS | ||
+ | |- | ||
+ | | titer (PFU/ml) | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |} | ||
+ | </center> | ||
==W/F lab== | ==W/F lab== | ||
Line 314: | Line 403: | ||
*Description of M13.1 phage: dual p8 | *Description of M13.1 phage: dual p8 | ||
*Description of MDS strain: MDS42 | *Description of MDS strain: MDS42 | ||
− | *Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. | + | *Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. We used the 10^-8 dilution data to calculate the three PFU/ml values. |
====Plaque numbers==== | ====Plaque numbers==== | ||
Line 355: | Line 444: | ||
|- | |- | ||
| titer (PFU/ml) | | titer (PFU/ml) | ||
− | | | + | | 1.74e12 PFU/ml |
− | | | + | | ND |
− | | | + | | 1.13e12 PFU/ml |
− | | | + | | 2.25e12 PFU/ml |
|} | |} | ||
</center> | </center> | ||
Line 367: | Line 456: | ||
*Description of MDS strain: | *Description of MDS strain: | ||
*Any notes about titers presented here: | *Any notes about titers presented here: | ||
+ | ====Plaque numbers==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS 43 | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS 43 | ||
+ | |- | ||
+ | | 10-6 | ||
+ | | 2400 | ||
+ | | 1632 | ||
+ | | 17 | ||
+ | | 27 | ||
+ | |- | ||
+ | | 10-8 | ||
+ | | 78 | ||
+ | | 43 | ||
+ | | 68 | ||
+ | | 25 | ||
+ | |- | ||
+ | | 10-10 | ||
+ | | 2 | ||
+ | | 2 | ||
+ | | 31 | ||
+ | | 49 | ||
+ | |} | ||
+ | </center> | ||
+ | |||
+ | ====Titer==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS43 | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS43 | ||
+ | |- | ||
+ | | titer (PFU/ml) | ||
+ | | 2.4E11 PFU/ml | ||
+ | | 1.63E11 PFU/ml | ||
+ | | 1.7E9 PFU/ml | ||
+ | | 2.7E9 PFU/ml | ||
+ | |} | ||
+ | </center> | ||
+ | |||
===Blue Team=== | ===Blue Team=== | ||
− | *Team members: | + | *Team members: Matthew Loper and Kat Pak |
*Description of M13.1 phage: | *Description of M13.1 phage: | ||
− | *Description of MDS strain: | + | *Description of MDS strain: MDS43 |
− | *Any notes about titers presented here: | + | *Any notes about titers presented here: negative control showed 62 plaques so there was probably contaimination |
+ | |||
===Yellow Team=== | ===Yellow Team=== | ||
*Team members: | *Team members: |
Latest revision as of 15:55, 15 June 2015
All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]
Contents
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ||||
10-8 | ||||
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
T/R lab
Green Team
- Team members: Arjun Naskar, Patrick Alayon
- Description of M13.1 phage: p3F17Ag
- Description of MDS strain: MDS60
- Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen.
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~1000 | ND | 0(uneven) | 0 |
10-8 | 0 (uneven) | ND | 0 | 0 |
10-10 | 0 | ND | 0 | 0 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Purple Team
- Team members:Phillip Samayoa, Justin Tan
- Description of M13.1 phage: p7p9 unstuff
- Description of MDS strain: MDS41
- Any notes about titers presented here: no phage control has plaques
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~500 | ND | 20 | 5 |
10-8 | 20 | ND | 3 | 5 |
10-10 | ~100 | ND | 0 | 5 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Red Team
- Team members:Rosa Alvarez, Vivian Hernandez
- Description of M13.1 phage:p9-YFP
- Description of MDS strain:MDS42
- Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | fully lysed | ND | 0 | 0 |
10-8 | ~1000 | ND | 0 | 0 |
10-10 | ~150 | ND | 0 | 0 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Pink Team
- Team members:Roshini Zachariah and Juliana Rotter
- Description of M13.1 phage: p3F17Ag
- Description of MDS strain: MDS60
- Any notes about titers presented here:negative control had no plaques as expected
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~100 | ND | 0 | 0 |
10-8 | 0 | ND | 0 | 0 |
10-10 | 0 | ND | 0 | ND |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Blue Team
- Team members: Augusto Tentori and Jessica Keenan
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:M13K07 control not grown in parallel
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~1000 | ND | ~1000 | ~1500 |
10-8 | 16 | ND | 10 | 42 |
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Yellow Team
- Team members:Rebecca Kusko and Jessie Wang
- Description of M13.1 phage:p8BAP
- Description of MDS strain: MDS60
- Any notes about titers presented here:
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~100 | ND | 0 | 0 |
10-8 | 9 | ND | 0 | 0 |
10-10 | 0 | ND | 0 | 1 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
W/F lab
Green Team
- Team members: Hao Ding and Neha Batra
- Description of M13.1 phage: p8::BAP
- Description of MDS strain: MDS12
- Any notes about titers presented here: Did not get culture for MDS12xM13.1, ctrl showed no plaques (no contamination)
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS 12 | phage = M13.1, strain = MG1655 | |
10-6 | >800 | >800 | 14 |
10-8 | 74 | 63 | 1 (maybe) |
10-10 | 1 (maybe) | 0 | 0 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | |
titer (PFU/ml) | 7.6e11 PFU/ml | 6.3e11 PFU/ml | 1.4e9 PFU/ml |
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Red Team
- Team members: Scott Pollom and Spenser Skates
- Description of M13.1 phage: dual p8
- Description of MDS strain: MDS42
- Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. We used the 10^-8 dilution data to calculate the three PFU/ml values.
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | too many | ND | 800 | too many |
10-8 | 174 | ND | 113 | 225 |
10-10 | 350 | ND | 1 | 231 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) | 1.74e12 PFU/ml | ND | 1.13e12 PFU/ml | 2.25e12 PFU/ml |
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS 43 | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS 43 | |
10-6 | 2400 | 1632 | 17 | 27 |
10-8 | 78 | 43 | 68 | 25 |
10-10 | 2 | 2 | 31 | 49 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS43 | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS43 | |
titer (PFU/ml) | 2.4E11 PFU/ml | 1.63E11 PFU/ml | 1.7E9 PFU/ml | 2.7E9 PFU/ml |
Blue Team
- Team members: Matthew Loper and Kat Pak
- Description of M13.1 phage:
- Description of MDS strain: MDS43
- Any notes about titers presented here: negative control showed 62 plaques so there was probably contaimination
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here: