Difference between revisions of "20.109(F07): M13.1 and MDS titers"
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Revision as of 20:48, 5 December 2007
All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]
Contents
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ||||
10-8 | ||||
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
T/R lab
Green Team
- Team members: Arjun Naskar, Patrick Alayon
- Description of M13.1 phage:
- Description of MDS strain: MDS60
- Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen.
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~1000 | ND | 0(uneven) | 0 |
10-8 | 0 (uneven) | ND | 0 | 0 |
10-10 | 0 | ND | 0 | 0 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here: no phage control has plaques
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ||||
10-8 | ||||
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Red Team
- Team members:Rosa Alvarez, Vivian Hernandez
- Description of M13.1 phage:p9-YFP
- Description of MDS strain:MDS42
- Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | fully lysed | ND | 0 | 0 |
10-8 | ~1000 | ND | 0 | 0 |
10-10 | ~150 | ND | 0 | 0 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Blue Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:M13K07 control not grown in parallel
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~1000 | ND | ~1000 | ~1500 |
10-8 | 16 | ND | 10 | 42 |
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
W/F lab
Green Team
- Team members: Hao Ding and Neha Batra
- Description of M13.1 phage: p8::BAP
- Description of MDS strain: MDS12
- Any notes about titers presented here: Did not get culture for MDS12xM13.1, ctrl showed no plaques (no contamination)
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS 12 | phage = M13.1, strain = MG1655 | |
10-6 | >800 | >800 | 14 |
10-8 | 74 | 63 | 1 (maybe) |
10-10 | 1 (maybe) | 0 | 0 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | |
titer (PFU/ml) | 7.6e11 PFU/ml | 6.3e11 PFU/ml | 1.4e9 PFU/ml |
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Red Team
- Team members: Scott Pollom and Spenser Skates
- Description of M13.1 phage: dual p8
- Description of MDS strain: MDS42
- Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. We used the 10^-8 dilution data to calculate the three PFU/ml values.
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | too many | ND | 800 | too many |
10-8 | 174 | ND | 113 | 225 |
10-10 | 350 | ND | 1 | 231 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) | 1.74e12 PFU/ml | - | 1.13e12 PFU/ml | 2.25e12 PFU/ml |
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Blue Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here: