Difference between revisions of "20.109(F07): Transmission electron microscopy"
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(New page: {{Template:20.109(F07)}} ==Introduction== *TEM *TEM grid *interp of what's visualized ==Protocols== ===Part 1: TEM sample preparation=== # Retrieve your dialyzed sammple from the beaker of...) |
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# Retrieve your dialyzed sammple from the beaker of water and very carefully remove the top clip. The tubing will be slippery and you will be very sad if it slips out of your hand and spills. | # Retrieve your dialyzed sammple from the beaker of water and very carefully remove the top clip. The tubing will be slippery and you will be very sad if it slips out of your hand and spills. | ||
# Use your P1000 to sip the sample out of the tubing, squeezing the liquid that's in the tubing toward the tip of the P1000. Place your iridium-coated phage (= nanowires) in an eppendorf tube. You will use only a small aliquot of this solution today but you will need it next time as well so <b> be sure to give the rest of your nanowire sample to the teaching faculty before you leave.</b> | # Use your P1000 to sip the sample out of the tubing, squeezing the liquid that's in the tubing toward the tip of the P1000. Place your iridium-coated phage (= nanowires) in an eppendorf tube. You will use only a small aliquot of this solution today but you will need it next time as well so <b> be sure to give the rest of your nanowire sample to the teaching faculty before you leave.</b> | ||
− | # Place 15 ul of your nanowire solution on the <b> shiny, bright side </b> of the TEM grid that you have balanced in the specialized tweezers. | + | # Place 15 ul of your nanowire solution on the <b> shiny, bright side </b> of the TEM grid that you have balanced in the specialized tweezers. [[Image:Macintosh_HD-Users-nkuldell-Desktop-tweezersTEMgrid.jpg|300 px]] |
# Allow the nanowires to settle onto the grid undisturbed for 30' | # Allow the nanowires to settle onto the grid undisturbed for 30' | ||
# Remove the droplet from the grid with your P200 set to 50 ul | # Remove the droplet from the grid with your P200 set to 50 ul |
Revision as of 01:17, 27 July 2007
Contents
Introduction
- TEM
- TEM grid
- interp of what's visualized
Protocols
Part 1: TEM sample preparation
- Retrieve your dialyzed sammple from the beaker of water and very carefully remove the top clip. The tubing will be slippery and you will be very sad if it slips out of your hand and spills.
- Use your P1000 to sip the sample out of the tubing, squeezing the liquid that's in the tubing toward the tip of the P1000. Place your iridium-coated phage (= nanowires) in an eppendorf tube. You will use only a small aliquot of this solution today but you will need it next time as well so be sure to give the rest of your nanowire sample to the teaching faculty before you leave.
- Place 15 ul of your nanowire solution on the shiny, bright side of the TEM grid that you have balanced in the specialized tweezers.
- Allow the nanowires to settle onto the grid undisturbed for 30'
- Remove the droplet from the grid with your P200 set to 50 ul
- Wash the grid by placing then immediately removing 15 ul of sterile H2O onto the grid.
- Dry the grid by very gently touching the edge of the grid to a piece of blotting paper in a petri dish then place the grid (shiny side up) onto the paper to transport to the TEM facility.
Part 2: TEM
DONE!