Difference between revisions of "20.109(S08): TA notes for module 1"
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'''Day of Lab:''' | '''Day of Lab:''' | ||
+ | |||
+ | *Students Benchtop | ||
+ | :*LB in 15mL conical tube | ||
+ | :*LB+Amp plates (5 per group, so at least 30 per lab) | ||
+ | :*1mL 3M NaAc | ||
+ | :*Ice buckets with ice | ||
+ | :*15mL conical tube with 100% EtOH in ice | ||
+ | :*Check spreaders, refill EtOH beakers and alcohol burners | ||
+ | |||
+ | *Instructors Benchtop | ||
+ | :*Ice bucket with ligation buffer | ||
+ | :*tRNA | ||
+ | :*2x 50mL conical tube with 70% EtOH | ||
+ | :*pCX-EGFP transformation control | ||
+ | :*Midway through lab, thaw supercomp cells (~400-500ul/group, need 12 tubes of ~200ul each for class of 12) | ||
+ | |||
*Quiz (prepared by TA). | *Quiz (prepared by TA). | ||
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===Agarose Gel=== | ===Agarose Gel=== | ||
− | # DNA gel: 1% agarose gel in 1X TAE, 1 g agarose, 100mL 1X TAE, 2 | + | # DNA gel: 1% agarose gel in 1X TAE, 1 g agarose, 100mL 1X TAE, 2 |